Sialidase Sp Alpha-(2-3)

- Overview
  - α(2-3) Sialidase Sp cleaves exclusively the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-6) or α(2-8) linkages or on branched α(2-3) linkages. To cleave all non-reducing terminal sialic acid residues including branched sialic acids (linked to an internal residue) from complex carbohydrates and glycoproteins, use α(2-3,6,8,9) Sialidase Au (E-S001).
    
    Contents:
    Sialidase Sp in 50 mM sodium phosphate, pH 7.5
    5x Reaction Buffer 250 mM sodium phosphate, pH 6.0
    
    Please contact us at for specific academic pricing.
    
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    Glycosidases
- Properties
  - Name
    
    Neuraminidase, N-acetylneuraminate glycohydrolase, Exo-α-sialidase
    
    Storage
    
    Store enzyme at 4°C.
    
    Activity
    
    10 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.18
    
    * For research use only
- Applications
  - Application
    
    Glycan Release
- Reference
  - 1. Corfield, A. P., H. Higa, J. C. Paulson and R. Schauer. The specificity of viral and bacterial sialidases for alpha(2-3) and alpha(2-6)-linked sialic acids in glycoproteins. Biochim Biophys Acta 744: 121-12 6 (1983).
    2. Dwek, R. A., C. J. Edge, D. J. Harvey, M. R. Wormald and R. B. Parekh. Analysis of glycoprotein-associated oligosaccharides. Ann Rev Biochem 62: 65-100 (1993).
    3. Kobata, A. Use of endo- and exoglycosidases for structural studies of glycoconjugates. Anal Biochem 100: 1-14 (1979).
    4. Glasgow, L R., J. C . Paulson and R. L. Hill. Systematic purification of five glycosidases from Streptococcus pneumoniae. J. Biol Chem 252: 8615-8623 (1977).
    5. Kelly, R. T., D. Greiff and S. Farmer. Neuraminidase activity in Diplococcus pneumoniae. J Bacteriol 91: 601-3 (1965).
    6. Prime, S., J. Dearnley , A. M. Venton, R. B. Parekh and C. J. Edge. Oligosaccharide sequencing based on exo- and endoglycosidase digestion and liquid chromatographic analysis of the products. J Chromatogr A 720: 263-274 (1996).

Documents

Specsheet

CofA

MSDS

Sialidase Sp Alpha-(2-3)

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