Sialate O-acetylesterase kit

Sialate O-acetylesterase kit

Catalog Number:
E001088477LUD
Mfr. No.:
LZ-ACASE-KIT
Price:
$687
  • Size:
    50 µL
    Quantity:
    Add to Cart:
      • Overview
        • Acetyl esterase (sialate-O-acetylesterase) is a recombinant protein from Tannerella forsythia, ATCC 43037 strain, expressed in Escherichia coli. The enzyme removes acetyl groups attached via an O- group, mainly 9-, 8- and 7-. It can be used for monitoring of diacetylation of sialic acids on products such as erythropoietin (EPO)

          Contents:
          LudgerZyme Acetyl Esterase (Supplied in PBS pH7.5 buffer containing 10 mM Tris-HCl)
          LudgerZyme Acetyl Esterase RXN 10x buffer (500 mM sodium acetate pH5.5)

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Source
          Recombinant from Tannerella forsythia in E. Coli.
          Storage
          Store at 4°C. Protect from sources of heat and light. When stored correctly, the enzyme should be stable for 24 months from date of purchase. Exposure to ambient temperatures (20 - 26°C) over 3 days does not result in a reduction of enzymatic activity.

          More Information

          Database Link

          * For research use only

      • Applications
        • Application
          Glycan Release
          Application Description
          Acetyl esterase (sialate-O-acetylesterase) can be used to remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins.
      • Reference
        • 1. Phansopa C, Kozak RP, Liew LP, Frey AM, Farmilo T, Parker JL, Kelly DJ, Emery RJ, Thomson RI, Royle L, Gardner RA, Spencer DI, Stafford GP. Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase. Biochem J. 2015 Dec 1;472(2):157-67. doi: 10.1042/BJ20150388. Epub 2015 Sep 16.
          2. Thomson RI, Gardner RA, Strohfeldt K, Fernandes DL, Stafford GP, Spencer DIR, Osborn HMI. Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Content. Anal Chem. 2017 Jun 20;89(12):6455-6462. doi: 10.1021/acs.analchem.7b00353. Epub 2017 Jun 9.

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