Beta-(1 -4)-galactosidase

- Overview
  - β Galactosidase from Streptococcus pneumoniae releases only β(1-4) linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. β(1-4) galactose is by far the most common linkage found in N-linked oligosaccharides. For other galactosidase linkages, β(1-3,4,6)-Galactosidase (E-BG02) from Bovine testes is recommended. The enzyme is as active on tetraantennary oligosaccharides as on disaccharides containing β(1-4)-linked galactose. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose. Up to 100 µg of asialofetuin can be completely β(1-4)-degalactosylated in less than 1 hour using 3 mU of enzyme.
    Contents:
    β-(1-4) Galactosidase in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
    5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).
    
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    Glycosidases
- Properties
  - Purification
    
    Each lot of β Galactosidase is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
    
    Activity
    
    >3 U/mL
    
    * For research use only
- Applications
  - Application
    
    Glycan Release
- Reference
  - 1. Glasgow, LR., J.C. Paulson and R.L. Hill. Systematic purification of five glycosidases from Streptococcus pneumonia. J. Biol Chem 252: 8615- 8623(1977).
    2. Kobata, A. Use of endo- and exoglycosidases for structural studies of glycoconjugates. Anal Biochem 100: 1- 14 (1979).
    3. Prime, S. J. Dearnley, A.M. Venton, R.B. Parekh and C.J. Edge. Oligosaccharide sequencing based on exo- and endoglycosidase digestion and liquid chromatographic analysis of the products. J Chromatogr A 720: 263-274 (1996)
    4. Dwek, R.A. , C.J. Edge, D.J. Harvey, M.R. Wormald and R.B. Parekh. Analysis of glycoprotein-associated oligosaccharides. Ann Rev Biochem 62: 65-100.

Documents

Specsheet

CofA

MSDS

Beta-(1 -4)-galactosidase

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Documents

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