Name: Recombinant Human IL-6 GMP Protein, CF Best Seller GMP
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 20.9 kDa
    N-terminal Sequence Analysis: Pro29-Val-Pro-Pro-Gly-Glu-Asp-Ser-Lys-Asp
    SDS-PAGE: 20-21 kDa, under reducing conditions.
    Host Cell Protein: <0.5 ng per µg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    Host Cell DNA: <0.0015 ng of DNA per µg of protein when tested by PCR.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Interleukin 6 (Alternate Names: BSF-2, BSF2, IFNB2, IL6, MGI-2A)
    
    Source
    
    E. coli
    
    Purification
    
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.10 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 100-200 μg/mL in sterile PBS.
    
    Activity
    
    Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.
    The ED50 for this effect is 0.2‑0.8 ng/mL.
    The specific activity of Recombinant Human IL-6 is >1.0 x 108 IU/mg, which is calibrated against the human IL‑6 WHO International Standard (NIBSC code: 89/548).
    
    More Information
    
    UniProt
    
    P05231.1
    
    Gene ID
    
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

Recombinant Human IL-6 GMP Protein Bioactivity GMP-grade Recombinant Human IL‑6 (Catalog # 206‑GMP) stimulates proliferation of T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 0.2‑0.8 ng/mL.

Recombinant Human IL-6 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-6 (Catalog # 206-GMP) was resolved with SDS-PAGE under reducing conditions (R) and visualized by silver staining, showing a single band at 21 kDa.

Recombinant Human IL-6 GMP Protein, CF Best Seller GMP

Recombinant Human IL-6 GMP Protein, CF Best Seller GMP

More Information

Recombinant Human IL-6 GMP Protein Bioactivity GMP-grade Recombinant Human IL‑6 (Catalog # 206‑GMP) stimulates proliferation of T1165.85.2.1 mouse plasma­cytoma cell line. The ED50 for this effect is 0.2‑0.8 ng/mL.

Recombinant Human IL-6 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-6 (Catalog # 206-GMP) was resolved with SDS-PAGE under reducing conditions (R) and visualized by silver staining, showing a single band at 21 kDa.

Recombinant Human IL-6 GMP Protein, CF Best Seller GMP

Recombinant Human IL-6 GMP Protein, CF Best Seller GMP

More Information

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Recombinant Human IL-6 GMP Protein Bioactivity GMP-grade Recombinant Human IL‑6 (Catalog # 206‑GMP) stimulates proliferation of T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 0.2‑0.8 ng/mL.