Name: Recombinant Human GM-CSF GMP Protein, CF Best Seller GMP
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 14.5 kDa
    N-terminal Sequence Analysis: Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr
    SDS-PAGE: 14 kDa, reducing conditions
    Host Cell Protein: < 0.5 ng per µg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Granulocyte Macrophage Growth Factor (Alternate Names: Granulocyte Macrophage Growth Factor)
    
    Source
    
    E. coli
    
    Purification
    
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in PBS.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.10 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 100-200 μg/mL in PBS.
    
    Activity
    
    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
    The ED50 for this effect is 6-30 pg/mL.
    The specific activity of recombinant human GM-CSF is >1.0 x 107 IU/mg, which is calibrated against the human GM-CSF WHO International Standard (NIBSC code: 88/646).
    
    More Information
    
    UniProt
    
    CSF2
    
    Gene ID
    
    1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

Recombinant Human GM-CSF GMP Protein Bioactivity GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) stimulates pro-liferation of TF‑1 human erythroleukemic cell line. The ED50 is 6-30 pg/mL.

Recombinant Human GM-CSF GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Recombinant Human GM-CSF GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP). The major peak corresponds to the calculated molecular mass, 14478 Da. The minor peak at 14673 Da is a matrix-associated artifact of the MALDI-TOF.

Recombinant Human GM-CSF GMP Protein, CF Best Seller GMP

Recombinant Human GM-CSF GMP Protein, CF Best Seller GMP

More Information

Recombinant Human GM-CSF GMP Protein Bioactivity GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) stimulates pro-liferation of TF‑1 human erythroleukemic cell line. The ED50 is 6-30 pg/mL.

Recombinant Human GM-CSF GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Recombinant Human GM-CSF GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP). The major peak corresponds to the calculated molecular mass, 14478 Da. The minor peak at 14673 Da is a matrix-associated artifact of the MALDI-TOF.

Recombinant Human GM-CSF GMP Protein, CF Best Seller GMP

Recombinant Human GM-CSF GMP Protein, CF Best Seller GMP

More Information

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