Name: Recombinant Human IL-4 GMP Protein, CF GMP
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 15.1 kDa
    N-terminal Sequence Analysis: Met-His25-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu
    SDS-PAGE: 13 kDa, under reducing conditions.
    Host Cell Protein: <0.100 ng per μg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    Host Cell DNA: <0.00150 ng per µg of protein when tested by PCR.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Interleukin 4 (Alternate Names: BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12)
    
    Source
    
    E. coli
    
    Purification
    
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. The molecular weight by mass spectrometry is 15079 Da ± 10 Da.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.10 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 500 μg/mL in PBS.
    
    Activity
    
    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
    The ED50 for this effect is 0.0400-0.320 ng/mL.
    
    The specific activity of GMP Recombinant Human IL-4 is >1.0x107 IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).
    
    More Information
    
    UniProt
    
    P05112.1
    
    Gene ID
    
    3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

Recombinant Human IL-4 Protein Bioactivity Comparison. Equivalent bioactivity of GMP (BT-004-GMP) and Animal-Free (BT-004-AFL) grades of Recombinant Human IL-4 as measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Green & Orange, respectively).

Recombinant Human IL‑4 GMP Protein SDS-PAGE. 2 μg/lane of Recombinant Human IL‑4 GMP Protein (Catalog # BT-004-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13 kDa under reducing conditions.

Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-4 GMP Protein, CF GMP

More Information

Recombinant Human IL-4 Protein Bioactivity Comparison. Equivalent bioactivity of GMP (BT-004-GMP) and Animal-Free (BT-004-AFL) grades of Recombinant Human IL-4 as measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Green & Orange, respectively).

Recombinant Human IL‑4 GMP Protein SDS-PAGE. 2 μg/lane of Recombinant Human IL‑4 GMP Protein (Catalog # BT-004-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13 kDa under reducing conditions.

Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-4 GMP Protein, CF GMP

More Information

We Also Recommend

Recombinant Human GM-CSF GMP Protein, CF Best Seller GMP

Recombinant Human IL-7 GMP Protein, CF Best Seller GMP

Recombinant Human Wnt-3a GMP Protein, CF GMP