Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-4 GMP Protein, CF GMP

Catalog Number:
P001494855BIO
Mfr. No.:
BT-004-GMP
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    • Overview
      • Predicted Molecular Mass: 15.1 kDa
        N-terminal Sequence Analysis: Met-His25-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu
        SDS-PAGE: 13 kDa, under reducing conditions.
        Host Cell Protein: <0.100 ng per μg of protein when tested by ELISA.
        Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
        Host Cell DNA: <0.00150 ng per µg of protein when tested by PCR.

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    • Properties
      • Protein Name
        Interleukin 4 (Alternate Names: BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12)
        Source
        E. coli
        Purification
        >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. The molecular weight by mass spectrometry is 15079 Da ± 10 Da.
        Conjugate
        Unconjugated
        Formulation
        Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
        Storage
        Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
        A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
        1 month, 2 to 8 °C under sterile conditions after reconstitution.
        3 months, ≤ -20 °C under sterile conditions after reconstitution.
        Endotoxin
        <0.10 EU per 1 μg of the protein by the LAL method.
        Reconstitution
        Reconstitute at 500 μg/mL in PBS.
        Activity
        Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
        The ED50 for this effect is 0.0400-0.320 ng/mL.

        The specific activity of GMP Recombinant Human IL-4 is >1.0x107 IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).

        More Information

        UniProt
        P05112.1
        Gene ID
        3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

        * This product is intended for research use or further manufacturing only.

    • Applications
      • Application
        Bioactivity