Name: Recombinant Human Wnt-3a GMP Protein, CF GMP
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 37.4 kDa
    N-terminal Sequence Analysis: Ser19-Tyr-Pro-Ile-Trp-Trp-Ser-Leu-Ala-Val
    SDS-PAGE: 40 kDa, reducing conditions
    Host Cell Protein: < 40 ng per µg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Wingless-type MMTV Integration Site Family, Member 3a (Alternate Names: Wnt3a)
    
    Source
    
    CHO cells
    
    Purification
    
    >75%, by SDS-PAGE with silver staining, under reducing conditions.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.10 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 200 μg/mL in PBS.
    
    Activity
    
    Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
    The ED50 for this effect is 5-25 ng/mL.
    
    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
    The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
    Optimal concentrations should be determined by each laboratory for each application.
    
    More Information
    
    UniProt
    
    NP_149122.1
    
    Gene ID
    
    89780 (Human); 22416 (Mouse)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

Recombinant Human Wnt-3a GMP Protein, CF GMP

Recombinant Human Wnt-3a GMP Protein, CF GMP

More Information

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