Recombinant Human Wnt-3a GMP Protein, CF GMP

Recombinant Human Wnt-3a GMP Protein, CF GMP

Catalog Number:
P001494856BIO
Mfr. No.:
5036-GMP
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    • Overview

      • Predicted Molecular Mass: 37.4 kDa
        N-terminal Sequence Analysis: Ser19-Tyr-Pro-Ile-Trp-Trp-Ser-Leu-Ala-Val
        SDS-PAGE: 40 kDa, reducing conditions
        Host Cell Protein: < 40 ng per µg of protein when tested by ELISA.
        Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.

        Please contact us at for specific academic pricing.

    • Properties
      • Protein Name
        Wingless-type MMTV Integration Site Family, Member 3a (Alternate Names: Wnt3a)
        Source
        CHO cells
        Purification
        >75%, by SDS-PAGE with silver staining, under reducing conditions.
        Conjugate
        Unconjugated
        Formulation
        Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
        Storage
        Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
        A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
        1 month, 2 to 8 °C under sterile conditions after reconstitution.
        3 months, ≤ -20 °C under sterile conditions after reconstitution.
        Endotoxin
        <0.10 EU per 1 μg of the protein by the LAL method.
        Reconstitution
        Reconstitute at 200 μg/mL in PBS.
        Activity
        Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
        The ED50 for this effect is 5-25 ng/mL.

        Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
        The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
        Optimal concentrations should be determined by each laboratory for each application.

        More Information

        UniProt
        NP_149122.1
        Gene ID
        89780 (Human); 22416 (Mouse)

        * This product is intended for research use or further manufacturing only.

    • Applications
      • Application
        Bioactivity
Datasheet
MSDS

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