Name: Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF GMP (Animal-Free)
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 17 kDa
    N-terminal Sequence Analysis: Ala-Pro-Val-Arg-Ser-Leu-Asn-(Cys)-Thr-Leu Pro-Val-Arg-Ser-Leu-Asn-(Cys)-Thr-Leu-Arg
    Host Cell Protein: < 0.5 ng per µg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Interleukin 1 beta (Alternate Names: IL-1b, IL-1F2, IL1 beta, IL1B)
    
    Source
    
    E. coli
    
    Purification
    
    >97%, by SDS-PAGE with silver staining, under reducing conditions.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in PBS.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.01 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 100-200 μg/mL in PBS.
    
    Activity
    
    Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
    The ED50 for this effect is <12 pg/mL.
    
    The specific activity of recombinant human IL-1 beta is >5.0 x 107 IU/mg, which is calibrated against the human IL-1 beta WHO International Standard (NIBSC code: 86/680).
    
    More Information
    
    UniProt
    
    NP_000567.1
    
    Gene ID
    
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

Recombinant Human IL-1 beta/IL-1F2 GMP Protein Bioactivity GMP-grade Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-GMP) stimu-lates proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

Recombinant Human IL-1 beta/IL-1F2 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF GMP (Animal-Free)

Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF GMP (Animal-Free)

More Information

Recombinant Human IL-1 beta/IL-1F2 GMP Protein Bioactivity GMP-grade Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-GMP) stimu-lates proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

Recombinant Human IL-1 beta/IL-1F2 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF GMP (Animal-Free)

Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF GMP (Animal-Free)

More Information

We Also Recommend

Recombinant Human IFN-gamma GMP Protein, CF Best Seller GMP

Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-6 GMP Protein, CF Best Seller GMP