Name: Recombinant Human IFN-gamma GMP Protein, CF Best Seller GMP
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 16.9 kDa
    N-terminal Sequence Analysis: Met-Gln24-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu
    SDS-PAGE: 17 kDa, reducing conditions
    Host Cell Protein: <0.5 ng per µg of protein when tested by ELISA.
    Mycoplasma: <0.5 ng per µg of protein when tested by ELISA.
    Host Cell DNA: <0.5 ng per µg of protein when tested by ELISA.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Interferon gamma (Alternate Names: IFG, IFI, IFNG, IFNgamma)
    
    Source
    
    E. coli
    
    Purification
    
    >97%, by SDS-PAGE with silver staining, under reducing conditions.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.01 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 200 μg/mL in sterile, deionized water.
    
    Activity
    
    Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
    The ED50 for this effect is 0.15-0.75 ng/mL.
    The specific activity of recombinant human IFN-gamma is >2.0 x 107 IU/mg, which is calibrated against the human IFN-gamma Standard (NIBSC code: 87/586).
    
    More Information
    
    UniProt
    
    CAA31639.1
    
    Gene ID
    
    3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

Recombinant Human IFN-gamma GMP Protein Bioactivity GMP-grade Recombinant Human IFN-gamma (Catalog # 285-GMP) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.15-0.75 ng/mL.

Recombinant Human IFN-gamma GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IFN-gamma (Catalog # 285-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Recombinant Human IFN-gamma GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human IFN-gamma (Catalog # 285-GMP). The major peak corresponds to the calculated molecular mass, 16881 Da. The minor peak at 17099 Da is a matrix-associated artifact of the MALDI-TOF.

Recombinant Human IFN-gamma GMP Protein, CF Best Seller GMP

Recombinant Human IFN-gamma GMP Protein, CF Best Seller GMP

More Information

Recombinant Human IFN-gamma GMP Protein Bioactivity GMP-grade Recombinant Human IFN-gamma (Catalog # 285-GMP) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.15-0.75 ng/mL.

Recombinant Human IFN-gamma GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IFN-gamma (Catalog # 285-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Recombinant Human IFN-gamma GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human IFN-gamma (Catalog # 285-GMP). The major peak corresponds to the calculated molecular mass, 16881 Da. The minor peak at 17099 Da is a matrix-associated artifact of the MALDI-TOF.

Recombinant Human IFN-gamma GMP Protein, CF Best Seller GMP

Recombinant Human IFN-gamma GMP Protein, CF Best Seller GMP

More Information

We Also Recommend

Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-4 GMP Protein, CF GMP

Recombinant Human IL-6 GMP Protein, CF Best Seller GMP

Recombinant Human Wnt-3a GMP Protein, CF GMP