Name: Recombinant Human EGF GMP Protein, CF GMP (Animal-Free)
Price: Inquiry

- Overview
  - Predicted Molecular Mass: 6 kDa
    N-terminal Sequence Analysis: Met-Asn971-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser
    SDS-PAGE: 6 kDa, reducing conditions
    Host Cell Protein: <0.5 ng per µg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    Host Cell DNA: <0.0015 ng per µg of protein when tested by PCR.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Epidermal Growth Factor (Alternate Names: HOMG4, URG, Urogastrone)
    
    Source
    
    E. coli
    
    Purification
    
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. The result of the major peak from mass spectrometry analysis is 6347 Da, which corresponds to the calculated molecular mass of 6353 Da.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in PBS.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -20 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.10 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 200 μg/mL in PBS.
    
    Activity
    
    Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.
    The ED50 for this effect is 20‑100 pg/mL.
    The specific activity of Recombinant Human EGF is >8.0 x 105 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).
    
    More Information
    
    UniProt
    
    P01133.2
    
    Gene ID
    
    1950 (Human); 13645 (Mouse); 25313 (Rat)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

MSDS

Recombinant Human EGF GMP Protein SDS-PAGE 1 µg/lane of GMP-grade Recombinant Human EGF (Catalog # 236-GMP) was resolved by SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

Recombinant Human EGF GMP Protein Mass Spectrometry MALDI‑TOF analysis of GMP‑grade Recombinant Human EGF (Catalog # 236‑GMP). The major peak corresponds to the calculated molecular mass, 6353 Da. The minor peak at 6549 Da is a matrix-associated artifact of the MALDI-TOF.

Recombinant Human EGF GMP Protein, CF GMP (Animal-Free)

Recombinant Human EGF GMP Protein, CF GMP (Animal-Free)

More Information

Recombinant Human EGF GMP Protein SDS-PAGE 1 µg/lane of GMP-grade Recombinant Human EGF (Catalog # 236-GMP) was resolved by SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

Recombinant Human EGF GMP Protein Mass Spectrometry MALDI‑TOF analysis of GMP‑grade Recombinant Human EGF (Catalog # 236‑GMP). The major peak corresponds to the calculated molecular mass, 6353 Da. The minor peak at 6549 Da is a matrix-associated artifact of the MALDI-TOF.

Recombinant Human EGF GMP Protein, CF GMP (Animal-Free)

Recombinant Human EGF GMP Protein, CF GMP (Animal-Free)

More Information

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