Name: Recombinant Human BMP-4 GMP Protein, CF Best Seller GMP
Price: Inquiry

- Overview
  - Structure / Form: Disulfide-linked homodimer
    Predicted Molecular Mass: 12 kDa
    N-terminal Sequence Analysis: Identity was confirmed by mass spectrometry
    SDS-PAGE: 8-9 kDa, reducing conditions
    Host Cell Protein: <0.5 ng per μg of protein when tested by ELISA.
    Mycoplasma: Negative when tested in a ribosomal RNA hybridization assay.
    Host Cell DNA: <0.0015 ng per µg of protein when tested by PCR.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Bone Morphogenetic Protein 4 (Alternate Names: BMP2B, BMP2B1, BMP4, MCOPS6, OFC11)
    
    Source
    
    E. coli
    
    Purification
    
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
    
    Storage
    
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, ≤ -65 °C under sterile conditions after reconstitution.
    
    Endotoxin
    
    <0.10 EU per 1 μg of the protein by the LAL method.
    
    Reconstitution
    
    Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.
    
    Activity
    
    Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells.
    The ED50 for this effect is 3-21 ng/mL.
    
    More Information
    
    UniProt
    
    P12644.1
    
    Gene ID
    
    652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
    
    * This product is intended for research use or further manufacturing only.
- Applications
  - Application
    
    Bioactivity

Datasheet

MSDS

GMP-grade Recombinant Human BMP-4 (Catalog # 314E-GMP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.

2 μg/lane of GMP-grade Recombinant Human BMP-4 Protein (314E-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-9 kDa and 20-22 kDa, respectively.

Recombinant Human BMP-4 GMP Protein, CF Best Seller GMP

Recombinant Human BMP-4 GMP Protein, CF Best Seller GMP

More Information

GMP-grade Recombinant Human BMP-4 (Catalog # 314E-GMP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.

2 μg/lane of GMP-grade Recombinant Human BMP-4 Protein (314E-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-9 kDa and 20-22 kDa, respectively.

Recombinant Human BMP-4 GMP Protein, CF Best Seller GMP

Recombinant Human BMP-4 GMP Protein, CF Best Seller GMP

More Information

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