Protein SN38 Conjugation Kit

We offer this kit to label any Protein (MW ≥ 20KDa) with SN38, the active metabolite of irinotecan, for early stage screening of your antibody.
Irinotecan is on the World Health Organization's List of Essential Medicines, the most effective and safe medicines needed in a health system. SN38 is 1000-times more active than irinotecan, but is not generally used as a drug due to its poor water solubility. If it is conjugated to a protein or antibody, the protein/antibody can transport SN38 to the active site. This kit includes O-succinyl SN38 NHS ester, which can be coupled to the protein directly via surface amines in a single step. The product is then purified to remove any unreacted drug.

• Drug Information
Name: SN38 (7-ethyl-10-hydroxycamptothecin)
CAS number: 86639-52-3
Mechanism of action: Inhibition of topoisomerase I leads to inhibition of both DNA replication and transcription.
Medical usage: Pro-drug Irinotecan (brand name: Camptosar) is used for treatment of colon and small cell lung cancer

• Requirement for Protein
Amount: 6.67-20 nmol
MW: >20 KDa
Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
Protein has some lysine residues

• Key Features of this PerKit™
Offers a simple and easy way to label proteins with SN38 with minimum exposure to the chemotherapeutic drug
Releasable linkage
Fast and easy preparation: 6 h preparation with <1 h hands-on time
All reagents and supplies included for preparation and purification
DPR (drug protein ratio) with average 3 SN38 labeling per antibody
Included stabilizing buffer for long-term storage
More than 95% conjugated products (free of any unreacted drugs)

Please contact us at for specific academic pricing.

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