F(ab')2 MMAE Conjugation Kit (With VC-PAB Linkage)

This kit for labeling any F(ab')2 with monomethyl auristatin E (MMAE) using a valine-citruline p-aminobenzylcarbamate (VC-PAB) linker for early stage screening of your antibody.

This kit includes maleimide-activated VC-PAB-MMAE, which can be coupled directly to a thiol modified F(ab')2 through alkylation in a single step (similar method developed by Seattle Genetics: Sun et al. 2005, Bioconjugate Chem. 16, 1282-1290). The product is purified to remove any unreacted drug.

Kit is configured to conjugate 0.73 to 2.2 mg of one (CM11416x1) or three F(ab')2 (CM11416x3).

If you have a smaller amount of F(ab')2 or other customization needs, please select the customization radio button and/or fill out the information above.

• Drug Information
Name: Monomethyl auristatin E (MMAE) with Mal-Val-Cit-PAB linkage
CAS number: 646502-53-6
Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid, but cleaved by cathepsin B once inside the tumor cell, thus activating the antimitotic mechanism
Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities

• Requirement for F(ab')2
Preferably >90% pure by gel electrophoresis
Total amount: 0.73-2.2 mg protein content measured by UV

• Key Features of this PerKit™
Offers a simple and easy way for site specific labeling of F(ab')2 with MMAE with minimum exposure to the toxin
Cathepsin B cleavable VC-PAB (Ref. Doronina et. al. 2008, Bioconjugate Chem. 19, 1960-1963)
Fast and easy preparation: 6 h preparation and <2 h hands-on time
All reagents and supplies included for preparation and purification
Easy to control the DAR if used together with the Thiol Assay Kit with Purification (Product#: CM90005)

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