PlantTaq™ DNA Polymerase (Chlorophyll-resistant)

PlantTaq™ DNA Polymerase (Chlorophyll-resistant) is a genetically engineered, highly efficient, thermostable DNA polymerase that is suitable for DNA amplifications directly from plant samples.

This product is highly resistant to PCR inhibitors such as chlorophyll, polysaccharides and polyphenols present a variety of plant species such as corn, loofah, bell pepper, bean, soybean, pepper, cucumber, eggplant, and tomato, etc. Freshly collected leaves or seeds of these plants, either stored at 4°C or frozen at low temperature, can be added directly into PCR mix to amplify the target DNA without the need of DNA extraction and purification.

This product requires limited quantities of plant samples. In a 20μl PCR reaction, a leaf disc of 0.1-1mm diameter is usually sufficient for DNA amplification.

A pair of positive control primers for amplification of a 297bp DNA fragment of the highly conserved choloroplastic IRB18 gene in many plant species is provided in this kit to confirm the normal working of PCR system.

This product can amplify DNA fragments up to 5kb.
DNA fragments amplified by this product are 3'-dA tailed, which can be cloned into T vectors directly.
Source: Recombinant DNA polymerase expressed in E. coli.
Purity: free of DNA endonuclease and exonuclease, phosphatase, and RNase.
Enzyme storage buffer: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50%. (v/v) glycerol.
Inactivation or inhibition: Phenol chloroform extraction can inactivate the PlantTaq™ DNA Polymerase.
When using 20µl for each PCR reaction, D7248S and D7248M are sufficient for 200 and 1000 reactions, respectively.

Please contact us at for specific academic pricing.