pRD29B-luc Luciferase Reporter Plasmid

pRD29B-luc is a luciferase reporter plasmid developed for transient transfection of plant (e.g., Arabidopsis thaliana) protoplasts. This plasmid contains the ampicillin marker for bacterial selection.

The expression of the Luciferase gene in the pRD29B-luc vector is driven by the RD29B promoter. RD29B is an Arabidopsis thaliana gene with induced expression under dehydration, low temperature or high salt conditions to enhance plant resistance to these unfavorable stress conditions. RD29B promoter is a commonly used stress-inducible promoter under which the expression of luciferase can be greatly enhanced upon treatment with abscisic acid (ABA) (ST1751). The constitutive expression of GUS genes driven by the cauliflower mosaic virus 35S (CaMV35S) promoter increases the metabolic burden in plant, causes a huge waste of material and energy, and thus affects the normal growth and development of the plant. By contrast, the RD29B promoter initiates the expression of downstream genes only under stress conditions, avoiding the disadvantages of CaMV35S promoter.

There are several enzyme digestion sites in the upstream and the downstream of the RD29B promoter in pRD29B-luc plasmid. Therefore, the RD29B promote can be replaced with other promoters to investigate the activation of the promoter of interest.

The pRD29B-luc vector contains only the components required for transient expression in plants to ensure higher transformation efficiency of protoplasts. This vector cannot be used to obtain stable transgenic plants.

pRD29B-luc plasmid employs the nopaline synthase terminator (NOS-T) from Agrobacterium rhizobium to terminate the transcription by RNA polymerase and regulate the expression of target genes.

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