Protease Inhibitor Cocktail for Plant Cell and Tissue Extracts, 100X

Protease Inhibitor Cocktail for Plant Cell and Tissue Extracts (100X) is designed for protein extraction from plant cells and tissues. It contains 200mM AEBSF, 10mM Bestatin, 3mM E64, 2mM Leupeptin, 2mM Pepstatin A, and 500mM Phenanthroline in high-purity DMSO.

Many endogenous proteases and phosphatases are commonly present in plant cell or tissue extracts, which easily leads to degradation or de-modification of protein samples. Therefore, adding appropriate inhibitors is generally necessary for protein extraction.

This product is optimized for protein extraction from mammalian cells or tissues. It contains serine, cysteine and acidic protease inhibitors with broad inhibitory activity, and aminopeptidase inhibitors.

This product can effectively inhibit various proteases in conventional cell or tissue extracts, such as serine proteases, aminopeptidases, cysteine proteases, threonine and aspartic proteases, and metalloproteases. Among its components, AEBSF is a water-soluble irreversible inhibitor of serine protease, with an inhibition constant similar to that of PMSF and DFP. It can effectively inhibit trypsin, chymotrypsin, plasmin, kallikrein, thrombin, and other proteases. As a substitute for PMSF and DFP, AEBSF has lower toxicity, better water solubility and better aqueous stability. Bestatin is a reversible inhibitor of aminopeptidase. E64 is an irreversible inhibitor of cysteine proteases. Leupeptin is a reversible inhibitor of serine and cysteine proteases. Pepstatin A is a reversible inhibitor of aspartic proteases. Phenanthroline is a reversible inhibitor of metalloproteinase.

This product is usually sufficient for preparing 100ml of lysis buffer.

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