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Overview
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This personalized antibody peptide conjugation kit is designed to label any IgG antibody with any peptide containing a reactive Cys group using EMCS crosslinker. The user supplies the antibody and peptide. The kit includes EMCS, which can be coupled first to the antibody to introduce the maleimide groups on the surface, and then the maleimide groups react with the Cys of the peptide to form the conjugates. The product is purified to remove any unreacted peptides.
This kit provides materials to perform one (CM32402x1) or three (CM32402x3) conjugations.
• Requirement for Cys peptide
Amount: 0.6 µmol
HPLC purified and lyophilized. Please make sure there is no reducing reagents, such as DTT are present. The Cys peptide should be stored at -80 degrees Celsius.
HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
• Requirement for antibody (IgG)
Amount: 3 mg (protein content)
Preferably >90% pure by gel electrophoresis
• Key Features of this PerKit™
Offers a simple and easy way to label IgG with peptide
Fast and easy preparation: 4 h preparation and <2 h hands-on time
Peptide loading is adjustable
All reagents and supplies included for preparation to purification
>95% conjugated products (free of or <5% unreacted peptide)Please contact us at for specific academic pricing.
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Overview