PerKit™ KLH Universal Small Molecule Acid Conjugation Kit

Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, and multi-subunit protein that contains chelated copper of non-heme origin. This personalized KLH conjugation kit is designed to work with any small molecule containing a carboxylic acid (-COOH) functional group. Using the kit components, the customer converts the carboxylic acid to an activated Sulfo N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid.

This kit provides materials to perform one KLH labeling. Total amount of KLH included: 5 mg.

• Requirement for Small Molecule
Preferably contains only one carboxylic acid (-COOH)
Total amount: ≥ 1.8 µmol
Preferably > 90% pure
Absence of primary or secondary amine (-NH2) groups
The presence of the hydroxide (-OH) group will not affect the labeling

• Key Features of this PerKit™
Offers a simple and easy way to label KLH with any small molecule containing carboxylic acid
Fast and easy preparation: 4 hr preparation and <30 minutes hands-on time
All reagents and supplies included for preparation and purification
Over 90% pure conjugated products by SEC

Please contact us at for specific academic pricing.