Perkit™ Antibody-Oligo Conjugation Kit

This personalized antibody-oligo conjugation kit is designed to work with any antibody and an amine-modified oligo. The user supplies their own antibody and amine-modified oligo (preferably a C6 amino modified oligo), which is readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a maleimide-oligo. Using the kit components, the customer converts some of the surface amines of the antibody to free thiol groups, followed by reaction of the maleimide-modified oligo to generate the conjugates. If there are any unreacted thiols on the antibody, they are subsequently capped. The final conjugate is desalted and buffer exchanged with PBS buffer.

This kit provides materials to perform one or three antibody oligo conjugations. Scale of the reaction: 0.25-1 mg antibody (protein content).

Note to previous users: If you purchased this kit previously, the current one is an upgraded and improved kit with slightly different components and workflow as the first and second-generation kit. This kit has multiple configurations for various lengths of oligos and can be used to conjugate 4−15 nano-mole (nmol) of oligo.

• Requirement for antibody:
Amount: 0.25-1 mg IgG needed per reaction
Preferably >90% pure by gel electrophoresis

• Requirement for amine-oligo:
Amount: 4-15 nmol oligo content as measured by UV
HPLC purified with amino-oligo content ≥90% by C18 HPLC.
≥5-mer. (oligos longer than 60-mer may result in lower loading and high impurities)
Note: You can purchase HPLC purified C6 amino-modified oligo from standard oligo manufacturers up to 90-mer length for this kit preparation. It is highly recommended that customers analyze and quantify oligos prior to using them.

• Key Features of this Antibody-Oligo Conjugation Kit:
Offers a convenient way to prepare antibody−oligo conjugate with heterobifunctional crosslinking reagents
Optimized precise loading with an average two oligos per antibody for high performance
Preparation can be done in a day (less than 8 hours) with various break points
≥90% conjugate if the quality of the oligo meets above standards
All reagents and supplies included for preparation to purification

Please contact us at for specific academic pricing.