pAAVtri-U6-shRNA-CMV-EGFP

pAAVtri-U6-ShRNA-CMV-EGFP plasmid is designed to produce AAV-shRNA vectors, in which the shRNA is under the control of the U6 Pol III promoter. Users can clone their shRNA into this plasmid by replacing the shRNA-scramble cassette between the HindIII and MluI sites. The CMV-EGFP expression cassette, located downstream of the U6-ShRNA cassette, allows for monitoring transfection efficiency. To generate AAV-shRNAs (AAV-U6-shRNA-CMV-EGFP) with the desired AAV serotypes, users can co-transfect this plasmid with the pRCap plasmid (which carries the AAV rep and cap genes) and the mini-pHelper-1.0 plasmid (which supplies adenoviral E2A, E4orf6, and VA RNA functions) into HEK 293T cells, using our AAVtri AAV production system.

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