pAAVtri-CMV-EGFP-miR30-shRNA

pAAVtri-CMV-EGFP-miR30-shRNA plasmid is designed to produce AAV-shRNA vectors, in which the shRNA is embedded into the miR30 expression cassette located at the EGFP 3'UTR, under the control of the CMV promoter. Users can clone their shRNA into this plasmid by replacing the shRNA-scramble cassette between the XhoI and EcoRI sites. To generate AAV-shRNAs (AAV-CMV-EGFP-miR30-shRNA) with the desired AAV serotypes, users can co-transfect this plasmid with the pRCap plasmid (which carries the AAV rep and cap genes) and the mini-pHelper-1.0 plasmid (which supplies adenoviral E2A, E4orf6, and VA RNA functions) into HEK 293T cells, using the AAVtri AAV production system.

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