pAAVone-AAV7-CMV-EGFP-2.8kb

pAAVone-AAV7-CMV-EGFP-2.8kb is a pAAVone plasmid used to produce AAV7 Reference (Full) and AAV7 Reference (Byproduct-Empty) using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV7), and a 2.8kb single-strand AAV-CMV-EGFP genome. AAV7-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV7 is a naturally occurring serotype of adeno-associated virus that is part of the same clade as AAV1 and AAV6. While less commonly used compared to other serotypes like AAV2, AAV8, or AAV9, AAV7 has shown potential in transducing a variety of tissues, particularly muscle and liver. It has been studied for its unique properties and could be considered for gene therapy applications where muscle or liver transduction is desired.

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Background

Our data showed that AAV7 delivers high levels of RNA and DNA across various tissues in C57BL/6 mice following intravenous (IV) injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.