pAAVone-AAV12-CMV-EGFP-2.8kb

pAAVone-AAV12-CMV-EGFP-2.8kb is a plasmid used to produce AAV12 Reference (Full) and AAV12 Reference (Byproduct-Empty) using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV-12), and a 2.8kb single-strand AAV-CMV-EGFP genome. AAV12-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAV12 is a relatively lesser-known serotype of adeno-associated virus that has been studied less extensively than more common serotypes like AAV2, AAV8, and AAV9. Our data demonstrated that AAV12 exhibits low RNA expression in the mouse (C57B6) tissues, but may have related high DNA level in kidney after IV injection.

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Background

Our data demonstrated that AAV12 exhibits low RNA expression in the mouse (C57B6) tissues, but may have related high DNA level in kidney after IV injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.