pAAVone-AAV4-CMV-EGFP-2.8kb

pAAVone-AAV4-CMV-EGFP-2.8kb is a pAAVone plasmid used to produce AAV4-CMV-EGFP using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV4), and a 2.8kb single-strand AAV-CMV-EGFP genome. AAV4-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAV4 is a lesser-known serotype of adeno-associated virus with a distinct tissue tropism compared to other AAV serotypes. It has a specific affinity for the airway epithelium and certain regions of the brain, such as the ependymal cells that line the brain's ventricular system. This makes AAV4 less commonly used in traditional gene therapy applications, but it has potential for targeting specific tissues where its tropism is beneficial.

Please contact us at for specific academic pricing.

Background

Our data demonstrated that AAV4 exhibits lung tropism in both RNA and DNA levels in the mouse (C57B6) after IV injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.