pAAVone-AAV11-CMV-EGFP-2.8kb

pAAVone-AAV11-CMV-EGFP-2.8kb is a pAAVone plasmid used to produce AAV11 Reference (Full) and AAV11 Reference (Byproduct-Empty) using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV11), and a 2.8kb single-strand AAV-CMV-EGFP genome. AAV11-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAV11 is relatively lesser-known serotype within the AAV family. Like other lesser-used AAV serotypes, it has been explored primarily in the context of understanding capsid diversity and tropism. AAV11 shares some similarities with other serotypes, but its tissue tropism and potential applications in gene therapy remain less characterized compared to more common serotypes such as AAV2, AAV8, and AAV9. Our data demonstrated that AAV11 exhibits high expression in the mouse lung at the RNA level and in the heart at the DNA level.

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Background

Our data demonstrated that AAV11 exhibits high expression in the mouse (C57B6) lung at the RNA level and in the heart at the DNA level after IV injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.