pAAVdual-hSyn-BFP

pAAVdual-hSyn-Cluc is used to produce AAV-hSyn-Cluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a hSyn promoter and an Cluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-hSyn-Cluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The hSyn promoter, also known as the human Synapsin 1 promoter, is a regulatory sequence used to drive gene expression specifically in neurons. Synapsin 1 is a neuron-specific phosphoprotein that plays a crucial role in the regulation of neurotransmitter release at synapses. The hSyn promoter is a neuron-specific regulatory element that drives strong and selective gene expression in neurons. It is widely used in neuroscience research, gene therapy, the development of transgenic models, and optogenetic studies. Its specificity to neurons makes it invaluable for targeting gene expression in the nervous system, facilitating research into neuronal function, connectivity, and neurological disorders.
Cypridina luciferase (Cluc), a bioluminescent reporter protein derived from the marine ostracod Cypridina noctiluca. Cluc emits light in the blue range when it catalyzes the oxidation of its substrate, Cypridina luciferin. This property makes Cluc an excellent tool for various biological research applications, particularly in non-invasive imaging and real-time monitoring of biological processes. AAV-Cluc is a powerful tool for researchers looking to perform sensitive, non-invasive imaging and real-time monitoring of gene expression and biological processes. Its high sensitivity and low background make it particularly useful for in vivo studies and applications where continuous, non-destructive observation is required.

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