pAAVdual-GFAP-BFP

pAAVdual-GFAP-Cluc is used to produce AAV-GFAP-Cluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a GFAP promoter and an Cluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-GFAP-Cluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The GFAP promoter refers to the regulatory sequence that controls the expression of the Glial Fibrillary Acidic Protein (GFAP) gene. GFAP is a key intermediate filament protein that is predominantly expressed in astrocytes, which are star-shaped glial cells in the central nervous system (CNS). The GFAP promoter is a glial-specific regulatory element that drives gene expression primarily in astrocytes within the central nervous system. It is a powerful tool in neuroscience research, gene therapy, and the development of transgenic models for studying astrocyte function, CNS development, and neurodegenerative diseases. Its specificity to astrocytes makes it invaluable for targeting gene expression in studies focused on glial cell biology, CNS health, and pathology.
Cypridina luciferase (Cluc), a bioluminescent reporter protein derived from the marine ostracod Cypridina noctiluca. Cluc emits light in the blue range when it catalyzes the oxidation of its substrate, Cypridina luciferin. This property makes Cluc an excellent tool for various biological research applications, particularly in non-invasive imaging and real-time monitoring of biological processes. AAV-Cluc is a powerful tool for researchers looking to perform sensitive, non-invasive imaging and real-time monitoring of gene expression and biological processes. Its high sensitivity and low background make it particularly useful for in vivo studies and applications where continuous, non-destructive observation is required.

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