pAAVdual-EF1a-BFP

pAAVdual-EF1a-BFP is used to produce AAV-EF1a-BFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a EF1a promoter and an BFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-EF1a-BFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The EF1a promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
Blue Fluorescent Protein (BFP) is a variant of Green Fluorescent Protein (GFP) that emits blue light when excited. BFP is generated by mutating the GFP protein, specifically by replacing the tyrosine residue at position 66 with histidine. This mutation shifts the emission peak from green to blue. BFP emits blue light with a peak around 440 nm when excited by UV light at approximately 380 nm.

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