pAAVdual-CaMKIIα-BFP

pAAVdual-CaMKIIα-BFP is used to produce AAV-CaMKIIα-BFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα promoter and a BFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα(0.36)-Cluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The CaMKIIα promoter refers to the regulatory sequence that controls the expression of the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is an essential enzyme predominantly expressed in the brain, especially in excitatory neurons of the forebrain, such as those found in the hippocampus and cortex. The CaMKIIα promoter is a neuron-specific regulatory element that drives gene expression in excitatory neurons of the forebrain, particularly in regions like the hippocampus and cortex. It is a critical tool in neuroscience research, transgenic model development, optogenetics, and potentially gene therapy. Its specificity to neurons involved in synaptic plasticity, learning, and memory makes it invaluable for studying the molecular and cellular mechanisms underlying these key brain functions.
Blue Fluorescent Protein (BFP) is a variant of Green Fluorescent Protein (GFP) that emits blue light when excited. BFP is generated by mutating the GFP protein, specifically by replacing the tyrosine residue at position 66 with histidine. This mutation shifts the emission peak from green to blue. BFP emits blue light with a peak around 440 nm when excited by UV light at approximately 380 nm.

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