pAAVdual-CaMKIIα(0.36)-BFP

pAAVdual-CaMKIIα(0.36)-BFP is used to produce AAV-CaMKIIα(0.36)-BFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα(0.36) promoter and a BFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα(0.36)-BFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.
Blue Fluorescent Protein (BFP) is a variant of Green Fluorescent Protein (GFP) that emits blue light when excited. BFP is generated by mutating the GFP protein, specifically by replacing the tyrosine residue at position 66 with histidine. This mutation shifts the emission peak from green to blue. BFP emits blue light with a peak around 440 nm when excited by UV light at approximately 380 nm.

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