pAAVdual-EF1a-Fluc

pAAVdual-EF1a-Fluc is used to produce AAV-EF1a-Fluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a EF1a promoter and an Fluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-EF1a-Fluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The EF1a promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
Firefly luciferase (Fluc) catalyzes the oxidation of its substrate, luciferin, in the presence of ATP and oxygen, producing light in the yellow-green spectrum. This bioluminescent reaction is widely used in biological research for real-time imaging, monitoring gene expression, and tracking various cellular processes. AAV-Fluc is a powerful tool for researchers who need to perform sensitive, non-invasive imaging and monitoring of gene expression and other cellular processes. Its high sensitivity, broad dynamic range, and compatibility with various research models make it an essential component of many bioluminescence-based assays and in vivo studies.

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