pAAVdual-CaMKIIα-Gluc

pAAVdual-CaMKIIα-Gluc is used to produce AAV-CaMKIIα-Gluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα promoter and an Gluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα-Gluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The CaMKIIα promoter refers to the regulatory sequence that controls the expression of the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is an essential enzyme predominantly expressed in the brain, especially in excitatory neurons of the forebrain, such as those found in the hippocampus and cortex. The CaMKIIα promoter is a neuron-specific regulatory element that drives gene expression in excitatory neurons of the forebrain, particularly in regions like the hippocampus and cortex. It is a critical tool in neuroscience research, transgenic model development, optogenetics, and potentially gene therapy. Its specificity to neurons involved in synaptic plasticity, learning, and memory makes it invaluable for studying the molecular and cellular mechanisms underlying these key brain functions.
Gaussia luciferase (Gluc) is a highly sensitive bioluminescent reporter protein commonly used in biological research to monitor gene expression, track cellular processes, or assess promoter activity in real time. By employing Gluc as a reporter, researchers gain the ability to effortlessly quantify the transduction efficiency of AAV vectors within target cells. The AAV-Gluc system allows researchers to perform in vivo and in vitro bioluminescent assays.

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