pAAVdual-CaMKIIα(0.36)-Gluc

pAAVdual-CaMKIIα(0.36)-Gluc is used to produce AAV-CaMKIIα(0.36)-Gluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα(0.36) promoter and an Gluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα(0.36)-Gluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.
Gaussia luciferase (Gluc) is a highly sensitive bioluminescent reporter protein commonly used in biological research to monitor gene expression, track cellular processes, or assess promoter activity in real time. By employing Gluc as a reporter, researchers gain the ability to effortlessly quantify the transduction efficiency of AAV vectors within target cells. The AAV-Gluc system allows researchers to perform in vivo and in vitro bioluminescent assays.

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