pAAVdual-EF1a-Cre

pAAVdual-EF1a-Cre is used to produce AAV-EF1a-Cre virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a EF1a promoter and an Cre reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-EF1a-Cre viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The EF1a promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
Cre recombinase is a site-specific DNA recombinase enzyme derived from the bacteriophage P1. Cre recombinase recognizes specific DNA sequences known as loxP sites and catalyzes the recombination between them. This recombination event can lead to the deletion, inversion, or translocation of the DNA segment flanked by loxP sites, depending on the orientation of the sites. AAV-Cre is a powerful tool for genetic research, enabling targeted manipulation of genes in specific tissues or at specific times. Its ability to drive site-specific recombination makes it an essential component of many gene editing, knockout, and lineage tracing studies.

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