pAAVdual-CaMKIIα(0.36)-Fluc

pAAVdual-CaMKIIα(0.36)-Fluc is used to produce AAV-CaMKIIα(0.36)-Fluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα(0.36) promoter and an Fluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα(0.36)-Fluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.
Firefly luciferase (Fluc) catalyzes the oxidation of its substrate, luciferin, in the presence of ATP and oxygen, producing light in the yellow-green spectrum. This bioluminescent reaction is widely used in biological research for real-time imaging, monitoring gene expression, and tracking various cellular processes. AAV-Fluc is a powerful tool for researchers who need to perform sensitive, non-invasive imaging and monitoring of gene expression and other cellular processes. Its high sensitivity, broad dynamic range, and compatibility with various research models make it an essential component of many bioluminescence-based assays and in vivo studies.

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