pAAVdual-GFAP-Fluc

pAAVdual-GFAP-Fluc is used to produce AAV-GFAP-Fluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a GFAP promoter and an Fluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-GFAP-Fluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The GFAP promoter refers to the regulatory sequence that controls the expression of the Glial Fibrillary Acidic Protein (GFAP) gene. GFAP is a key intermediate filament protein that is predominantly expressed in astrocytes, which are star-shaped glial cells in the central nervous system (CNS). The GFAP promoter is a glial-specific regulatory element that drives gene expression primarily in astrocytes within the central nervous system. It is a powerful tool in neuroscience research, gene therapy, and the development of transgenic models for studying astrocyte function, CNS development, and neurodegenerative diseases. Its specificity to astrocytes makes it invaluable for targeting gene expression in studies focused on glial cell biology, CNS health, and pathology.
Firefly luciferase (Fluc) catalyzes the oxidation of its substrate, luciferin, in the presence of ATP and oxygen, producing light in the yellow-green spectrum. This bioluminescent reaction is widely used in biological research for real-time imaging, monitoring gene expression, and tracking various cellular processes. AAV-Fluc is a powerful tool for researchers who need to perform sensitive, non-invasive imaging and monitoring of gene expression and other cellular processes. Its high sensitivity, broad dynamic range, and compatibility with various research models make it an essential component of many bioluminescence-based assays and in vivo studies.

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