pAAVdual-CaMKIIα-Fluc

pAAVdual-CaMKIIα-Fluc is used to produce AAV-CaMKIIα-Fluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα promoter and an Fluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα-Fluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The CaMKIIα promoter refers to the regulatory sequence that controls the expression of the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is an essential enzyme predominantly expressed in the brain, especially in excitatory neurons of the forebrain, such as those found in the hippocampus and cortex. The CaMKIIα promoter is a neuron-specific regulatory element that drives gene expression in excitatory neurons of the forebrain, particularly in regions like the hippocampus and cortex. It is a critical tool in neuroscience research, transgenic model development, optogenetics, and potentially gene therapy. Its specificity to neurons involved in synaptic plasticity, learning, and memory makes it invaluable for studying the molecular and cellular mechanisms underlying these key brain functions.
Firefly luciferase (Fluc) catalyzes the oxidation of its substrate, luciferin, in the presence of ATP and oxygen, producing light in the yellow-green spectrum. This bioluminescent reaction is widely used in biological research for real-time imaging, monitoring gene expression, and tracking various cellular processes. AAV-Fluc is a powerful tool for researchers who need to perform sensitive, non-invasive imaging and monitoring of gene expression and other cellular processes. Its high sensitivity, broad dynamic range, and compatibility with various research models make it an essential component of many bioluminescence-based assays and in vivo studies.

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