pAAVdual-TBG-BFP

pAAVdual-TBG-BFP is used to produce AAV-TBG-BFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a TBG promoter and a BFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. TBG-BFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The TBG promoter is derived from the thyroxine-binding globulin (TBG) gene, which is primarily expressed in the liver and encodes a key protein involved in thyroid hormone transport. In AAV applications, this promoter delivers strong, hepatocyte-specific expression with high durability, making it one of the most widely adopted regulatory elements in liver-directed gene therapy. It has been validated in both preclinical and clinical studies, supporting research in metabolic disorders, systemic protein replacement, and other hepatocyte-targeted therapies.
Blue Fluorescent Protein (BFP) is a variant of Green Fluorescent Protein (GFP) that emits blue light when excited. BFP is generated by mutating the GFP protein, specifically by replacing the tyrosine residue at position 66 with histidine. This mutation shifts the emission peak from green to blue. BFP emits blue light with a peak around 440 nm when excited by UV light at approximately 380 nm.

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