Name: Ofloxacin
Price: 162.00 USD

- Overview
  - Ofloxacin is a synthetic, broad-spectrum, second-generation fluoroquinolone antibiotic that is an analog of nalidixic acid. It was developed by Daiichi Seiyaku to be resistant to metabolic oxidation in the liver and inhibits the supercoiling activity of bacterial DNA gyrase, halting DNA replication. It is used to combat organisms causing urinary tract and respiratory infections.
    
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    Background
    
    Ofloxacin is a Cytochrome P-450 inhibitor and DNA Topoisomerase II inhibitor. Fluoroquinolone antibiotics target bacterial DNA gyrase, an enzyme which reduces DNA strain during replication. Because DNA gyrase is required during DNA replication, subsequent DNA synthesis and ultimately cell division is inhibited.
- Properties
  - CAS Number
    
    82419-36-1
    
    Molecular Formula
    
    C18H20FN3O4
    
    Molecular Weight
    
    361.37
    
    Appearance
    
    White crystalline powder
    
    Solubility
    
    Glacial Acetic Acid: SolubleMethanol: Slightly solubleWater: Slightly soluble
    
    Other Properties
    
    Source: Synthetic
    Assay: (On Dried Basis): 98.5-101.5%
    Loss on Drying: ≤0.2%
    Melting Point: 250-257°C
    Optical Rotation: -1° to +1°
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Ofloxacin is a broad-spectrum antibiotic targeting a wide range of Gram-positive and Gram-negative organisms.
    
    Microbiology Applications: Ofloxacin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    
    Haemophilus influenzae 0.016 µg/mL – 0.063 µg/mL
    Escherichia coli 0.063 µg/mL – 0.125 µg/mL
    
    Cancer Applications: Oflaxacin had an inhibitory effect on the proliferation of transitional cell carcinoma cell lines at high concentrations (>200 μg/ml). The effect may be related to impairment of telomerase activity by some unknown mechanism (Yamakuchi M et al, 1997).
    Ofloxacin inhibited proliferation and DNA synthesis of these 3 human TCC lines (CCSUP, T24, and J82 ) in vitro. Inhibition occurred in a concentration- and time-dependent manner. Inhibition of proliferation and DNA synthesis were assessed via MTT and tritiated thymidine assays (Seay, 1996).