Naïve Cells

Naïve Cells

Catalog Number:
BC01578978HOO
Mfr. No.:
NAC-0110; NAC-0111; NAC-0112; NAC-0113; NAC-0114; NAC-0115
Price:
$624
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      • Overview
        • Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).

          These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.

          Availability
          Fresh cells from naïve mice (no immunization or disease induction). Termination at age 8 to 18 weeks.
          Spleen cells from C57BL/6 mice (no antigen).
          Lymph node cells from C57BL/6 mice (no antigen).
          Spleen cells and lymph node cells from C57BL/6 mice (no antigen).
          Spleen cells from SJL mice (no antigen).
          Lymph node cells from SJL mice (no antigen).
          Spleen cells and lymph node cells from SJL mice (no antigen).

          Preparation
          Mice 8 to 14 weeks of age are acclimated to our facility for at least 7 days.

          Fresh cell products
          Cell suspensions are prepared and red blood cells in spleen are lysed.

          Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.

          Cells are shipped the same day for overnight delivery.

          Please contact us at for specific academic pricing.

      • Properties
        • Other Properties
          Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.
          Cells are supplied in 15 or 50 mL centrifuge tubes, in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).
          Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cell will be pooled in one tube with one cell count.
          Storage
          Fresh cells ship cold on gel packs in insulated packaging. Use or freeze within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight.

          * For Research Use Only.