gpMBP69-88/CFA Emulsion for EAE Induction in Lewis Rats

Emulsion supplied in pre-filled syringes, ready to use.

•Consistently induces EAE in female Lewis rats
EAE will develop in 90 to 100% of rats within 10 to 14 days of immunization. Maximum score for individual rats will typically be 3.0 to 3.5.

Each lot is tested in vivo and individually adjusted to ensure consistent disease induction.

•Eliminates tedious preparation of emulsions
Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.

•Saves time and rats in testing individual reagents
Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.

•Reduces your rodent facility's exposure to pathogen contamination
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your rodent facility.

Experimental autoimmune encephalomyelitis (EAE) is the model most commonly used to study efficacy of potential drugs for treatment of multiple sclerosis (MS).

The EAE model in Lewis rats has consistent disease onset and severity, as well as a short clinical course, making it a popular compound screen for potential efficacy in MS.

EAE in Lewis rats is characterized by paralysis and CNS inflammation. Demyelination is not typically observed. EAE is initiated by myelin-specific CD4+ T cells, with glia cells (microglia and astrocytes), B cells, macrophages, NK cells, dendritic cells, and mast cells all likely playing roles in disease development.

Most investigators immunize Lewis rats with gpMBP69-88 to induce EAE (cat. no. EIK-0115, without pertussis toxin). In this model rats fully recover by Day 20 after immunization.

It is possible to induce more severe EAE by administering pertussis toxin (PTX) to rats one day after immunization. If PTX is administered in addition to the antigen, 30% to 60% of rats will experience a relapse within 28 days from immunization. This provides an opportunity for study of mechanism of EAE development and relapses.

The Hooke Kit™ gpMBP69-88/CFA Emulsion is intended for Lewis rats aged 9 to 14 weeks and is supplied as an emulsion in syringes.

Please contact us at for specific academic pricing.