MOUSE α/β TCR RNA Kit

The Mouse α/β TCR RNA Kit is designed for unbiased amplification of TCR alpha and beta cDNA libraries using 5’RACE (Rapid Amplification of cDNA Ends) with unique molecular identifiers (UMI) incorporated within template switch oligo. 5’RACE UMI technique allows for advanced PCR and sequencing error correction, exact quantification of template cDNA molecules, and accurate normalization of samples for comparisons of repertoire diversity metrics.
The kit includes a set of reagents sufficient to prepare 24 TCR alpha and 24 TCR beta cDNA libraries starting from 24 RNA samples.
The kit allows to start with RNA derived from 100 to 0.5 million sorted/purified T-cells, from peripheral blood leukocytes, or from T cell-containing tissues (see Appendixes A and B for recommended RNA isolation procedures), and produces indexed ready-to-sequence-on-Illumina® libraries. Up to 24 samples can be processed in parallel.

MiXCR is a universal solution for fast and rigorous extraction and analysis of T-cell and B-cell receptor repertoire high-throughput sequencing data.

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