mini-pHelper-AAV2

mini-pHelper-AAV2 is a compact, optimized plasmid designed to produce AAV2 empty particles or AAV2 vectors. It integrates AAV2 Rep and AAV2 Cap genes into our mini-pHelper-1.0 backbone. Thus, it not only provides adenoviral helper functions (E2A, E4orf6, VA RNA), but also AAV helper functions (Rep and AAV2). When transfected into host cells alone, it facilitates the generation of truly-empty AAV2 particles without any genetic materials inside of capsids. Co-transfection mini-pHelper-AAV2 with pAAVtri-GOI enables high-efficiency production of recombinant AAV2 vectors carrying your gene of interest (GOI).

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