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Overview
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mini-pHelper-AAV-DJ is a compact, optimized plasmid designed to produce AAV-DJ empty particles or AAV-DJ vectors. It integrates AAV-DJ Rep and AAV-DJ Cap genes into our mini-pHelper-1.0 backbone. Thus, it not only provides adenoviral helper functions (E2A, E4orf6, VA RNA), but also AAV helper functions (Rep and AAV-DJ). When transfected into host cells alone, it facilitates the generation of truly-empty AAV-DJ particles without any genetic materials inside of capsids. Co-transfection mini-pHelper-AAV-DJ with pAAVtri-GOI enables high-efficiency production of recombinant AAV-DJ vectors carrying your gene of interest (GOI).
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Overview