mini-pHelper-AAV12

mini-pHelper-AAV12 is a compact, optimized plasmid designed to produce AAV12 empty particles or AAV12 vectors. It integrates AAV12 Rep and AAV12 Cap genes into our mini-pHelper-1.0 backbone. Thus, it not only provides adenoviral helper functions (E2A, E4orf6, VA RNA), but also AAV helper functions (Rep and AAV12). When transfected into host cells alone, it facilitates the generation of truly-empty AAV12 particles without any genetic materials inside of capsids. Co-transfection mini-pHelper-AAV12 with pAAVtri-GOI enables high-efficiency production of recombinant AAV12 vectors carrying your gene of interest (GOI).

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