Recommended applications, sorted by nucleic acid

METAFECTENE® PRO - DNA & RNA Transfection Reagent

Name: METAFECTENE® PRO - DNA & RNA Transfection Reagent
Price: 430.00 USD

Catalog Number:

TR02135771BIO

Mfr. No.:

T040-10; T040-20; T040-50

Price:

$430

Size:

Quantity:

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- Overview
  - METAFECTENE® PRO is the latest generation transfection reagent setting a new benchmark in mammalian cell transfection. METAFECTENE® PRO is a liposome based transfection reagent consisting of a mixture of polycationic and neutral lipids. Structural changes of the comprising cationic lipids results in higher efficiency and lower toxicity. Quality tested, multiple enhancement of efficiency can be obtained eminently using METAFECTENE® PRO, even with moderately hard or hard-to-transfect cell lines.
    
    Highlights
    ·Suitable for DNA, mRNA, siRNA and miRNA transfection as well as cotransfection of DNA and RNA
    ·Suitable for stable and transient transfection
    ·Suitable for adherend and suspension cells
    ·Minimized toxic effects
    ·Optimized transfection rates
    ·Free from serum inhibition
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    METAFECTENE® Lipofection Reagents
- Properties
  - Categories
    
    Mammalian Cell Line Transfection Reagent
    
    Other Properties
    
    Shelf Life: 1 year (from date of delivery)
    Assays: Up to 1500 (24 well) or up to 400 (6 well) per 1 ml reagent
    
    Storage
    
    4°C
    
    Shipping
    
    At room temperature
    
    * Only for research purposes in vitro, not intended for human or animal diagnostic, therapeutic or other clinical uses.
- Applications
  - Application
    
    Mammalian Cell Line Transfection
    
    Application Description
    
    Suitable for transient and stable transfection of mammalian cell lines and primary cells with DNA (plasmids, bacmide), RNA (mRNA, miRNA, and siRNA), and modified nucleic acids (e.g. antisense oligonucleotides).
    Application areas:
    ·The production of proteins, antibodies and virus (e.g. AAV, adenovirus, lentivirus)
    ·Cotransfectionens of various nucleic acids, genome editing (e.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown)
- Reference
  - "The DNase activity of Kaposi’s sarcoma-associated herpesvirus SOX protein serves an important role in viral genome processing during lytic replication." Uppal et al., 2019, Journal of virology, 93.8: e01983-18
    "MicroRNA-mediated down-regulation of NKG2D ligands contributes to glioma immune escape." Codo et al., 2014, Oncotarget, 5.17: 7651
    "Aspirin inhibition of prostaglandin synthesis impairs egg development across mosquito taxa." Al Baki et al., 2020, bioRxiv
    "DEAD-box RNA helicase DDX23 modulates glioma malignancy via elevating miR-21 biogenesis." Yin et al., 2015, Brain, 138.9: 2553-2570