MAPK Signaling Antibody Array

MAPK Antibody Array is designed for expression profiling of key proteins in MAP Kinase signaling cascades. It is suitable for total protein profiling in human cell lysates, tissue lysates, serum, or culture media. Each package contains two identical array slides for analyzing two samples, such as an untreated sample and a treatment sample.

The mitogen-activated protein kinase (MAPK) Antibody Array is an ELISA based antibody array for studying MAP kinase signaling cascades. It is designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features:
· Qualitative/semi-quantitative protein expression profiling with 60 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for two samples (untreated vs. treated)
· Featured Antibodies: 63 antibodies; 6 replicates per antibody
· Reactivity: Human
· Suitable Sample Type: Cell lysate | Tissue lysate | Serum | Culture Media
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
AKT1, AKT2, Akt3, A-RAF, ASK1 (MAP3K5), ASK2 (MAP3K6), ASK3 (MAP3K15), BRAF, c-RAF, CREB, Elk1, “ERK1 (MAPK3, p44-MAPK)”, “ERK2 (MAPK1, p42-MAPK)”, ERK3 (MAPK6), “ERK4 (MAPK4, p63MAPK)”, ERK7 (MAPK15), GSK3 alpha, GSK3 beta, HGK (MAP4K4), HSP27, JNK1 (MAPK8), JNK2 (MAPK9), JNK3 (MAPK10), KHS1 (MAP4K5), KHS2 (MAP4K3), LAMTOR3 (MAP2K1IP1), MAP2K4, MEK1 (MAP2K1), MEK2 (MAP2K2), MEK3 (MAP2K3), MEK5 (MAP2K5), MEK6 (MAP2K6), MEKK1 (MAP3K1), MEKK3 (MAP3K3), MEKK4 (MAP3K4), MINK (MAP4K6), MK2 (MAPKAPK2), MK3 (MAPKAPK3), MLK (MAP3K13), MLK1 (MAP3K9), MLK2 (MAP3K10), MLK3 (MAP3K11), MLK4 (MAP3KL4), MSK1 (RPS6KA5), mTOR, NF-kB p65, p38A (MAPK14), p38B (MAPK11), p38D (MAPK13), p38G (MAPK12), p44/42 MAPK, p53, p70S6K (RPS6KB1), p70S6K2 (RPS6KB2), PRAK (MAPKAPK5), RSK1 (RPS6KA1), RSK2 (RPS6KA3), RSK3 (RPS6KA2), RSK4 (RPS6KA6), RSKL1 (RPS6KC1), STAT1, TAB1 (MAP3K7IP1), TAK1 (MAP3K7)

Assay Outline:
The ELISA based MAPK Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.