Signal Transduction Antibody Array

Signal Transduction Array features 165 highly specific and well-characterized antibodies related to signal transduction. It's suitable for protein profiling in human cell lysates, tissue lysates, serum, or culture media. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Signal Transduction Antibody Array is a high-throughput ELISA based antibody array, designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features:
· Qualitative/semi-quantitative protein expression profiling with 165 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for two samples (untreated vs. treated)
· Featured Antibodies: 165 antibodies; 6 replicates per antibody
· Reactivity: Human
· Suitable Sample Type: Cell lysate | Tissue lysate | Serum | Culture Media
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
14.3.3 gamma, 14.3.3 Pan, 14-3-3 beta, Adenovirus, Adenovirus Fiber, Adenovirus Type 2 E1A, Adenovirus Type 5 E1A, ADP-ribosylation Factor, Alkaline Phosphatase, Alpha Fetoprotein, Alpha Lactalbumin, alpha-1-antichymotrypsin, alpha-1-antitrypsin, Amphiregulin, Amylin Peptide, Amyloid A, Amyloid A4 Protein Precursor, Amyloid Beta, Androgen Receptor, Bonzo/STRL33/TYMSTR, c-Abl, CD8, CDC25C, Chk1, c-JUN, CREB, CREB-Binding Protein, c-Src, DJ-1, DNA Ligase I, DNA Polymerase Beta, DNA Polymerase Gamma, DNA Primase(p49), DNA Primase(p58), DNA-PKcs, DP-2, DR5, Dysferlin, Dystrophin, Dystrophin, E2F-1, E2F-2, E2F-3, E2F-4, E2F-5, E3-binding protein, EGFR, EMA/CA15-3/MUC-1, Endostatin, Epithelial Membrane Antigen, Epithelial Specific Antigen, ER beta, ER Ca+2 ATPase2, ERCC1, ERK1, ERK2, Estriol, Estrogen Receptor, Ezrin/p81/80K/Cytovillin, Factor VIII Related Antigen, FADD, Fli-1, FLIP, FLT-1/VEGFR1, Flt-4, Fra2, FSH, FSH-b, Fyn, Gab-1, GCDFP-15, Granzyme B, Grb2, GRIP1, GSK-3, GSK3b, HDAC1, Heat Shock Factor 1, Heat Shock Factor 2, hsp27, hsp60, hsp70, 75/hsp75, hsp86, hsp84, Hepatic Nuclear Factor-3B, Hepatocyte, Hepatocyte Factor Homologue-4, Hepatocyte Growth Factor, HIF-1a, Histone H1, HPV 16, HPV 16-E7, IFN gamma, IgA, IGF-1R, IGF-I, IgM (m-Heavy Chain), IKKb, IL-2, IL-3, IL-30, IL-4, IL-5, IL-6, IL-10, IL-10R, IL17, Insulin, Insulin Receptor, Insulin Receptor Substrate-1, Int-2 Oncoprotein, Integrin beta5, Interferon-a(II), Interferon-g, IRAK, ITK, JKK1, Lck, Lck(p56lck), Leukotriene (C4,D4,E4), LewisA, LewisB, Maltose Binding Protein, MAP1B, MAP2a-b, MART-1, Mcl-1, Mek1, Mek2, Mek6, Mekk-1, Microphthalmia, Nck, NF kB/p50, NFkB/p65, NOS-i, NOS-u, P53, PAX-5, PDGF, PDGFR-a, PHAS-I, PHAS-II, Phospho-Ser/Thr/Tyr, Phosphotyrosine, Raf1, Raf-1(Phospho), Rhodopsin (Opsin), Ribonucleoprotein, SHP-1, SIM Ag, SIRP a1, Stat-1, Stat3, Stat5, Stat5a, Stat5b, Stat6, Superoxide Dismutase, Syk, TNFa, TTF-1

Assay Outline:
The ELISA based Signal Transduction Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.