Signaling Explorer Antibody Array

Signaling Explorer Antibody Array features 1358 unique antibodies covering 20 cell signaling pathways, ideal for high-throughput biomarker screening and discovery. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

The Signaling Explorer Antibody Array is a high-throughput ELISA based antibody array for qualitative/semi-quantitative protein expression profiling. Featuring 1358 antibodies, this array is designed for broad screening of protein level changes in multiple cell signaling pathways, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features:
· Qualitative/semi-quantitative protein expression profiling
· Suitable samples: cell lysates; frozen or FFPE tissue lysates; serum; culture media
· Glass based array with high specificity and low background
· Antibodies are covalently attached to 3D polymer coated glass slides
· Sensitive fluorescent detection

Specifications:
· Product Size: 2 array slides per package for analyzing two samples (control vs. treated)
· Number of Antibodies: 1358 antibodies; 2 replicates per antibody
· Target List: Antibody List
· Reactivity: Human
· Internal Controls: beta-actin; GAPDH; Negative controls
· Detection Method: Fluorescence |Compatible Scanners
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 theta, 14-3-3 zeta, 4E-BP1, 5-HT-1A, 5-HT-1F, 5-HT-2C, 5-HT-3A, 5-HT-4, 5-HT-5A, A1BG, A26C2/3, AARSD1, AASDHPPT, AATF, ABCA8, ABCB7, ABCD1, ABHD11, ABHD12, ABHD12B, ABHD14A, ABHD14B, ABHD4, ABL1, ACAD10, ACBD6, ACOT2, ACOT4, ACSL6, Actin-alpha-1, Actin-gamma2, Actin-pan, ACTL6A, ACTN 1/2/3/4, ACTN alpha-2/3, ACTR-1C, ACTR3, ACVL1, ADA2L, ADAM 17, ADAR1, ADCK1, ADCK2, ADCK3, ADCK5, ADCY4, ADCY5/6, ADCY7, ADCY8, ADD2, ADD3, ADH7, ADK, ADNP, ADPGK, ADRB1, Adrenergic Receptor alpha-2A, Adrenergic Receptor alpha-2B, Adrenergic Receptor alpha-2C, Aggrecan, AGR3, and more.

Assay Outline:
The ELISA based Cell Signaling Explorer Antibody Array platform involves four major steps:
1) Protein extraction with non-denaturing lysis buffer;
2) Biotinylation of protein samples;
3) Incubation of labeled samples with antibody array;
4) Detection by dye conjugated streptavidin.

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.