Phospho Explorer Antibody Array

Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and biological processes. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Protein phosphorylation plays an important role in cell signaling and metabolism. Phospho Explorer Antibody Array serves as a high-throughput, ELISA based, phosphorylation assay for qualitative protein phosphorylation profiling. It is designed for comparing normal samples to treated or diseased samples and identifying candidate biomarkers. This phospho antibody array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.

Key Features:
· Phosphorylation profiling with 1318 site-specific antibodies from over 30 signaling pathways
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for analyzing two samples (untreated vs. treated)
· Featured Antibodies: 1318 site-specific and phospho-specific antibodies; 2 replicates per antibody
· Reactivity: Human: 99% | Mouse: 92% | Rat: 66%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
14-3-3 theta/tau (Ser232), 14-3-3 zeta (Ser58), 14-3-3 zeta/delta (Ab-232), 14-3-3 zeta/delta (Thr232), 4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), Abl1 (Tyr204), Abl1 (Tyr412), ACC1 (Ser79), ACC1 (Ser80), ACK1 (Tyr284), AFX (Ser197), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT2 (Ser474), ALK (Tyr1507), ALK (Tyr1604), AMPK1 (Thr174), Amyloid beta A4 (Thr743/668), Androgen Receptor (Ser213), Androgen Receptor (Ser650), A-RAF (Tyr301/302), Arrestin-1 (Ab-412), Arrestin-1 (Ser412), ASK1 (Ser83), ASK1 (Ser966), ATF-1 (Ser63), ATF2 (Ab-112/94), ATF2 (Ser112/94), ATF2 (Ser62/44), ATF2 (Thr69/51), ATF2 (Thr71/53), ATF2 (Thr73/55), ATF4 (Ser245), ATP1A1/Na+K+ ATPase1 (Ser23), ATPase (Ser16), ATP-Citrate Lyase (Ser454), ATRIP (Ser68/72), AurB (Thr232), AurB (Tyr12), Aurora Kinase (Thr288), AXL (Tyr691), and more.

Assay Outline:
The ELISA based Phospho Explorer Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.